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定制阵列式合成引导 RNA 文库是实现 CRISPR 敲除筛选的一个好方法。它们规避了与慢病毒筛选相关的克隆和测序,同时还可整合到现有自动化工作流程中,从而能够加速您的研究。
Alt-R 定制 CRISPR gRNA 文库可用于所有 CRISPR 核酸酶,包括 Cas9、Cas12a、Cas13、主要编辑酶和其他替代系统。开发这些文库是为了满足对更好的CRISPR筛选解决方案的需求。它们是在 IDT 专有的高保真 RNA 生产平台上合成的化学修饰的向导 RNA,可提供高质量、可靠的快速交付 gRNA 文库。
特点
Options
设计
Predesigned, custom, user-provided
CRISPR systems
Cas9, Cas12a, Cas13, prime editing, and other alternative systems
保证得率
0.5 nmol, 2 nmol, 5 nmol, and custom normalized deliverables
Cas9 gRNA formats
2-part crRNA:tracrRNA complex and sgRNA
Custom lengths supported
30–150 nt
Chemical modifications
2’-O-methyl RNA, PS linkages, end-blocking Alt-R modifications
Plate types
96- & 384-well PCR, Deep-well, V-bottom, Echo, custom options available
Formulation options
Multi-guide per well; pooled by gene
Arrayed (single gRNA/well)
Custom formulations upon request
QC
Individual ESI/MS
Supporting reagents & functional analysis pipeline (optional)
WT Cas9, HiFi Cas9, Cas12a. and Cas12a Ultra
Glycerol-free options available in tubes or plates (ideal for robotics)
Electroporation Enhancers
rhAmpSeq™ CRISPR Analysis System (NGS-based on-/off-target editing analysis)
我们的 Alt-R CRISPR 酶有多种形式可供选择,原液大小高达 50 mg。可根据要求为所有产品提供更大的规格和批次匹配.
Alt-R CRISPR 酶
Cas protein
Available versions
核心优势
S.p. Cas9 Nuclease V3
Wild-type, 50% Glycerol
Wild-type, Glycerol-free
High Fidelity (HiFi)
Wild-type Fluorescent-fusionTargeting GC-rich regions
Low viscosity for high-throughput applications
Reduced off-target activity*
Fluorescent label for enrichment (GFP or RFP)
A.s. Cas12a (Cpf1) V3
野生型
UltraTargeting AT-rich regions
Increased on-target activity*
L.b. Cas12a (Cpf1)
Ultra
Increased on-target activity and low-temperature tolerance*


